Transfection Reagents
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT SET 4/REAGENT 1.5ML
DharmaFECT set of 4 transfection reagents, 1.5ml
Oz Biosciences STEM TRANSFECTION REG 1ML
Stem Transfection Reagent is a powerful reagent allowing multipotent stem cells transfection with high efficiency and very low toxicity. Its specific composition based on the TEE-technology allows transfecting embryonic and multipotent stem cells in presence of serum maintaining their undifferentiated stage and their capacities to differentiate.
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Fibroblasts
In addition to various cell type-specific kits for primary fibroblasts, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian fibroblast type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for fibroblasts.Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support Team Transfection efficiencies up to 90%Viabilities up to 98%Applications:Kit suited for fibroblasts from different mammalian species and various organs Already tested for macaque dermal fibroblasts bovine fibroblasts human colon myofibroblasts mouse lung fibroblasts etc.
Apexbio Technology LLC ARCA EGFP mRNA, 100ug (1mg/mL). Cas: MFCD: NA. Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells.
EGFP (enhanced green fluorescent protein) mRNA can express an enhanced green fluorescence once has been transfected into cells, which makes it always used as a direct detection reporter mRNA. You can observe the fluorescence at 509 nm. Our product had already been modified with ARCA with a high efficient co-transcriptional capping method, which means most of mRNA has a Cap 0 structure. It is a more mature mRNA than mRNA with no capping and maintain a more stable state which lead to higher expressing efficiency in transfected mammalian cells. ARCA obtain the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with mCAP. Other sizes are available. Please inqury us for quote.
HORIZON DISCOVERY BIOSCIENCES LIMITED DharmaFECT Set of 4 Transfection Reagents, 0.75 mL
An aliquot of each of the four DharmaFECT formulations for siRNA/microRNA transfection optimization studies. Useful for determination of the best reagent for your cells and particular experimental conditions.
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT 1 TRANSFECTION REAG
DharmaFECT 1 Transfection Reagent
Lonza Walkersville Human Macrophage Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human macrophages.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Macrophages0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Human Stem Cell Nucleofector Kit 2
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell Nucleofector Kit 2 is one of our two kits suited for efficient transfection of primary human stem cells. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Stem Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes